Related Experiment Video
Updated: Apr 27, 2026
A Precise Pathogen Delivery and Recovery System for Murine Models of Secondary Bacterial Pneumonia
Published on: September 21, 2019
Eosinophils and respiratory virus infection: a dual-standard curve qRT-PCR-based method for determining virus
Caroline M Percopo1, Kimberly D Dyer, Kendal A Karpe
1Inflammation Immunobiology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 9000 Rockville Pike, 20892, Bethesda, MD, USA.
Abstract:
Several lines of investigation have indicated a role for eosinophilic leukocytes in limiting virus infectivity and promoting virion clearance. We have established a respiratory virus infection model with pneumonia virus of mice (PVM; family Paramyxoviridae), a natural mouse pathogen that replicates the more severe forms of human disease elicited by the phylogenetically related respiratory syncytial virus (RSV). In this chapter, we present a rapid and highly reproducible dual-standard curve qRT-PCR based method for quantitative detection of PVM replication in mouse lung tissue. We have used this assay to evaluate eosinophil-mediated antiviral host defense in mouse models of cytokine and antigen-driven eosinophilic inflammation.
