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Updated: Apr 27, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Effects of several acetylated chitooligosaccharides on antioxidation, antiglycation and NO generation in erythrocyte
Chang-Mei Zhang1, Shu-Han Yu1, Liang-Shuan Zhang1
1Pharmacy College of Harbin Medical University, Xuefu Street 194, Nangang District, Harbin 150081, PR China.
Abstract:
Three kinds of chitooligosaccharides (COS) with different degrees of deacetylation were prepared and named MD90, MD70 and MD50, respectively. Antioxidation, antiglycation and nitric oxide (NO) promotion in erythrocyte of these samples were investigated. The results showed that COS, especially MD90 had obviously inhibitory effects on oxidation and glycation. In addition, MD90 displayed stronger effect on increasing endogenous NO content than both MD70 and MD50, whose degrees of deacetylation were lower. The results indicated that amino group in COS has a certain effect on the activities of COS. As COS have a conformed activity to treat diabetes, the results of this study may be meaningful for further understanding the mechanism of the action.
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