Jove
Visualize
Contact Us

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K
Microbial Biosensors01:17

Microbial Biosensors

88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of multivariate health index for tropical seagrass meadows: a case study from Pulicat Lagoon, India.

Environmental monitoring and assessment·2026
Same author

Fluorescence-based dual detection of chlortetracycline and Pb<sup>2+</sup> using nitrogen/phosphorus co-doped carbon quantum dots.

RSC advances·2025
Same author

Corn cob nanocellulose packaging for increasing the shelf life of food products.

International journal of biological macromolecules·2024
Same author

Effect of gamma-ray irradiated reduced graphene oxide (rGO) on environmental health: An in-vitro and in-vivo studies.

Environmental pollution (Barking, Essex : 1987)·2022
Same author

Electrochemical impedimetric immunosensor based on stabilized lipid bilayer-tethered WS<sub>2</sub>@MWCNT for the sensitive detection of carcinoembryonic antigen.

Mikrochimica acta·2022
Same author

Benthic foraminifera as an environmental proxy for pollutants along the coast of Chennai, India.

Chemosphere·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 27, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

16.6K

Diatom-based label-free optical biosensor for biomolecules.

S Viji1, M Anbazhagi, N Ponpandian

  • 1Department of Nanoscience and Technology, Bharathiar University, Coimbatore, 641 046, Tamilnadu, India.

Applied Biochemistry and Biotechnology
|July 4, 2014
PubMed
Summary
This summary is machine-generated.

This study demonstrates that functionalized diatom frustules from Amphora sp. can detect bovine serum albumin (BSA) using optical biosensing. The amine-functionalized biosilica shows enhanced photoluminescence, enabling sensitive and label-free protein detection.

More Related Videos

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

9.2K
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
07:22

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

16.6K
Label-free Single Molecule Detection Using Microtoroid Optical Resonators
08:53

Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

9.2K
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

Published on: May 3, 2011

14.3K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Biosensing

Background:

  • Diatom frustules are unicellular algae with intricate biosilica shells (frustules).
  • These frustules possess nanoscale porous structures and reactive silanol groups, making them suitable for biomolecule detection.
  • The specific diatom Amphora sp. was chosen for its unique frustule architecture.

Purpose of the Study:

  • To functionalize Amphora sp. diatom frustules for the detection of bovine serum albumin (BSA).
  • To investigate the optical properties of functionalized frustules for biosensing applications.
  • To establish a quantitative analytical method for label-free protein detection.

Main Methods:

  • Functionalization of diatom frustules using 3-aminopropyltriethoxysilane (APES).
  • Characterization using Field Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared Spectroscopy (FTIR).
  • Photoluminescence (PL) spectroscopy to analyze optical changes upon BSA interaction.

Main Results:

  • FESEM confirmed the nanoscale patterned surface of the diatom frustules.
  • FTIR spectra verified the successful amine functionalization.
  • Amine-functionalized frustules exhibited a tenfold enhancement in PL intensity.
  • A significant quenching of PL intensity at 445 nm was observed upon BSA interaction, indicating successful detection.
  • The detection limit for BSA was determined to be 3 × 10⁻⁵ M.

Conclusions:

  • Functionalized Amphora sp. diatom frustules serve as an effective platform for optical biosensing.
  • The developed method allows for quantitative, label-free detection of proteins like BSA.
  • This approach holds promise for developing novel analytical tools in diagnostics and research.