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Updated: Apr 27, 2026

Detection of Bacteria Using Fluorogenic DNAzymes

Detection of Bacteria Using Fluorogenic DNAzymes

Published on: May 28, 2012

Flow-based enzymatic ligation by sortase A.

Rocco L Policarpo¹, Hansol Kang, Xiaoli Liao

¹Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 (USA).

Angewandte Chemie (International Ed. in English)

|July 4, 2014

Summary

This summary is machine-generated.

This study introduces a novel flow-based sortagging platform using immobilized sortase A. This method enables efficient protein modification with low nucleophile concentrations, overcoming limitations of traditional methods.

Keywords:

flow chemistry immobilization microreactor protein modification sortase A

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Published on: February 5, 2020

Area of Science:

Biochemistry
Chemical Biology
Protein Engineering

Background:

Sortase-mediated ligation (sortagging) is a key protein modification technique.
Traditional sortagging requires a large excess of nucleophiles to drive reactions and prevent side reactions.
This excess can be costly and complicate purification.

Purpose of the Study:

To develop an efficient flow-based sortagging platform.
To enable sortagging at low nucleophile concentrations.
To overcome limitations of solution-phase batch sortagging.

Main Methods:

Immobilization of sortase A enzyme within a microreactor.
Development of a continuous flow system for protein ligation.
Testing the platform with various protein reaction partners.

Main Results:

Achieved efficient sortagging at significantly reduced nucleophile concentrations.
Demonstrated the platform's versatility with multiple reaction partners.
Successfully generated a complex protein bioconjugate not feasible with batch methods.

Conclusions:

The developed flow-based sortagging platform offers an efficient and versatile alternative to traditional methods.
This approach allows for reduced reagent usage and enables novel bioconjugate synthesis.
Immobilized enzyme technology in microreactors shows great promise for protein engineering.