Simultaneous determination of 11 bioactive compounds in Jaeumganghwa-tang by high performance liquid
Bo-Ra Yun1, Jin Bae Weon1, Jiwoo Lee1
1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, Republic of Korea.
Background:
Jaeumganghwa-tang (JEGH) is a traditional Korean herbal medicine for the treatment of chronic bronchitis, nephritis and diabetes mellitus.
Objective:
A high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed for simultaneous determination of 11 major compounds such as 5- hydroxymethylfurfural, mangiferin, paeoniflorin, nodakenin, naringin, hesperidin, decursinol, berberine, glycyrrhizin, atractylenolide III and decursin, in JEGH.
Materials And Methods:
The separation was conducted on Shishedo C18 column with gradient elution of 0.1% trifluoroacetic acid-acetonitrile. Detection of wavelength was set at 205, 250, 280 and 330 nm.
Results:
The developed analysis showed a good linearity (R (2) >0.9997). The range of limit of detection and limit of quantification were observed from 0.04 to 0.43 and from 0.11 to 1.30, respectively. The intra- and inter-day test relative standard deviations (RSD) were less than 3% and the accuracy was 95.98-108.44%. The recoveries were between 92.75% and 109.19% and RSD range of recoveries was measured from 0.52% to 2.78%.
Conclusion:
This HPLC-DAD method can be successfully applied for simultaneous determination of 11 major compounds in JEGH samples.
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