Sensitive flow-injection spectrophotometric analysis of bromopride

Area of Science:

• Analytical Chemistry
• Spectrophotometry
• Flow Injection Analysis

Background:

• Bromopride is a widely used pharmaceutical agent.
• Accurate determination of bromopride in various matrices is crucial for quality control and clinical monitoring.
• Existing methods may require complex sample preparation or lack sensitivity.

Purpose of the Study:

• To develop and validate a novel flow injection spectrophotometric method for the direct determination of bromopride.
• To optimize the method using experimental design for enhanced efficiency.
• To assess the applicability of the method in pharmaceutical formulations and biological fluids.

Main Methods:

• Utilizing a flow injection analysis (FIA) system with merging zones.
• Employing the reaction between bromopride and p-dimethylaminocinnamaldehyde (p-DAC) in an acidic medium with sodium dodecyl sulfate (SDS).
• Spectrophotometric detection of the violet product at λmax=565nm, with optimization via experimental design.

Main Results:

• The Beer-Lambert law was obeyed over a concentration range of 3.63×10⁻⁷ to 2.90×10⁻⁵ molL⁻¹ (r=0.9999).
• Limits of detection and quantification were determined to be 1.07×10⁻⁷ and 3.57×10⁻⁷ molL⁻¹, respectively.
• Successful application to pharmaceutical formulations and human urine with high recovery rates (99.6-101.2% and 98.6-102.1%).

Conclusions:

• The proposed flow injection spectrophotometric method provides a sensitive, rapid, and accurate means for bromopride determination.
• The method eliminates the need for sample pretreatment, simplifying analysis.
• This technique is suitable for routine analysis of bromopride in quality control and biological sample monitoring.