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Related Experiment Video

Updated: Apr 27, 2026

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

Stool methylated DNA markers decrease following colorectal cancer resection--implications for surveillance.

John B Kisiel¹, Tracy C Yab, William R Taylor

¹Division of Gastroenterology and Hepatology, Mayo Clinic, 200 First Street Southwest, Rochester, MN, 55905, USA, kisiel.john@mayo.edu.

Digestive Diseases and Sciences

|July 5, 2014

Summary

This summary is machine-generated.

Methylated gene markers in stool decrease after colorectal cancer (CRC) resection, returning to normal levels unless disease persists. Postoperative stool testing can aid in CRC surveillance.

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Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

Area of Science:

Oncology
Molecular Diagnostics
Gastroenterology

Background:

Colorectal cancer (CRC) molecular changes are detectable in stool DNA.
These molecular markers may persist after tumor removal surgery.

Purpose of the Study:

To investigate if methylated gene markers in stool normalize post-CRC resection.
To assess the clinical utility of these markers in postoperative surveillance.

Main Methods:

Studied stool samples from 22 CRC patients before and after surgery, and 80 controls.
Assayed methylated NDRG4 and BMP3 genes using quantitative real-time target and signal amplification.
Dichotomized results using 95% specificity cutoffs.

Main Results:

Median levels of methylated NDRG4 and BMP3 significantly decreased post-resection (p = 0.003 and p < 0.0001).
93% of CRC cases with elevated preoperative levels normalized after surgery (p = 0.0002).
One case with rising methylated NDRG4 showed recurrent CRC.

Conclusions:

Methylated gene markers in stool clear after CRC resection if no disease is present.
Postoperative stool marker levels are informative for CRC surveillance.