Inhibitory effects of (-)-loliolide on cellular senescence in human dermal fibroblasts

Area of Science:

• Gerontology
• Cell Biology
• Natural Product Chemistry

Background:

• Cellular senescence plays a dual role in tumor suppression and progression, impacting tissue repair, aging, and age-related diseases.
• Targeting aging processes presents a promising strategy for preventing and treating various age-related conditions.
• Human dermal fibroblasts (HDFs) are a key cell type for studying skin aging and senescence.

Purpose of the Study:

• To investigate the inhibitory effects of (-)-loliolide, isolated from Polygonum aviculare, on cellular senescence in HDFs.
• To determine if (-)-loliolide can reverse or mitigate established senescence markers.
• To explore the potential of (-)-loliolide as an anti-aging agent.

Main Methods:

• Extraction and purification of (-)-loliolide from Polygonum aviculare.
• Induction of cellular senescence in HDFs using adriamycin treatment.
• Assessment of senescence markers including senescence-associated β-galactosidase (SA-β-gal) activity, p21 protein levels, and reactive oxygen species (ROS).
• Evaluation of (-)-loliolide's effect on SA-β-gal activity in HDFs undergoing replicative senescence.

Main Results:

• (-)-Loliolide significantly reduced SA-β-gal activity in adriamycin-induced senescent HDFs.
• The compound decreased the levels of p21 protein and reactive oxygen species in senescent HDFs.
• (-)-Loliolide also attenuated SA-β-gal activity in HDFs experiencing replicative senescence.
• These results demonstrate a clear anti-senescence effect of (-)-loliolide.

Conclusions:

• (-)-Loliolide effectively rescues HDFs from both induced and replicative cellular senescence.
• The findings suggest (-)-loliolide's potential utility in developing anti-aging dietary supplements and cosmetics.
• (-)-Loliolide may serve as a valuable agent for ameliorating tissue aging and age-associated diseases.