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Updated: Aug 3, 2026

A Method for Microinjection of Patiria minata Zygotes
Published on: September 1, 2014
Pertussis toxin inhibits 1-methyladenine-induced maturation in starfish oocytes
1Department of Biology, University of Southern California, Los Angeles 90089.
Abstract:
Starfish oocytes injected with pertussis toxin (3-6 micrograms/ml) or its catalytically active A-subunit (1 microgram/ml) did not undergo germinal vesicle breakdown in response to 1-methyladenine (1-10 microM). The pertussis block could be bypassed by transfer of cytoplasm that contained maturation-promoting factor (MPF). After insemination, pertussis-blocked, MPF-rescued oocytes underwent cortical vesicle exocytosis and cleavage. These results suggest the involvement of a pertussis sensitive G-protein in the pathway coupling 1-methyladenine action at the cell surface to the reinitiation of meiosis.
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