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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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Applications of Molecular Taxonomy01:20

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Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
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Related Experiment Video

Updated: Apr 27, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next generation sequencing technology: Advances and applications.

H P J Buermans1, J T den Dunnen1

  • 1Leiden Genome Technology Center, Leiden University Medical Center, Postbus 9600, 2300 RC Leiden, The Netherlands.

Biochimica Et Biophysica Acta
|July 5, 2014
PubMed
Summary

Next Generation Sequencing (NGS) offers unprecedented views into complex biological samples. This technology is revolutionizing biomedical research by providing powerful molecular insights.

Keywords:
ApplicationsBasic technologyNanoporeNext generation sequencingSequence by synthesisSingle molecule sequencing

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Area of Science:

  • Genomics and Molecular Biology
  • Biomedical Research Applications

Background:

  • Next Generation Sequencing (NGS) has advanced significantly due to molecular biology and engineering innovations.
  • Parallelization of sequencing reactions has dramatically increased sequence output per run.
  • Current NGS platforms provide unparalleled insights into complex RNA and DNA mixtures.

Approach:

  • Review of technical aspects of commercially available NGS platforms.
  • Discussion of template generation and sequencing reactions.
  • Exploration of upcoming NGS technologies.

Key Points:

  • NGS acts as a molecular microscope, impacting diverse biomedical research fields.
  • Detailed review of current NGS platform technologies.
  • Future trends in NGS technology are considered.

Conclusions:

  • NGS is a transformative technology in biomedical research.
  • The chapter provides a technical overview and future outlook of NGS.
  • NGS implementations across biomedical research are summarized.