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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Related Experiment Video

Updated: Apr 27, 2026

Test Samples for Optimizing STORM Super-Resolution Microscopy
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Technical review: types of imaging-direct STORM.

Ellen Jensen, David J Crossman

    Anatomical Record (Hoboken, N.J. : 2007)
    |July 5, 2014
    PubMed
    Summary

    Direct stochastic optical reconstruction microscopy (dSTORM) offers super-resolution imaging of cellular structures at 20 nm resolution. This technique utilizes single-fluorophore localization without needing an activator, compatible with common fluorophores.

    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Cell Biology

    Background:

    • Microscopy innovations in the 1990s broke the diffraction limit for far-field imaging.
    • Super-resolution microscopy emerged in 2006 via single-fluorophore localization.
    • Single-molecule spectroscopy advanced nanometer-scale optical microscopy.

    Purpose of the Study:

    • To provide an overview of direct stochastic optical reconstruction microscopy (dSTORM).
    • To explain the principles and applications of dSTORM.
    • To discuss the advantages and disadvantages of dSTORM.

    Main Methods:

    • Utilizes high-precision localization of single fluorophores.
    • Achieves super-resolution imaging without an activator fluorophore.
    • Compatible with a wide range of conventional fluorophores.

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    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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    Main Results:

    • Enables visualization of cellular structures with approximately 20 nm resolution.
    • Represents a significant advancement in single-molecule super-resolution imaging.
    • Offers a distinct approach to nanometer-scale optical microscopy.

    Conclusions:

    • dSTORM is a powerful technique for high-resolution cellular imaging.
    • Its compatibility and lack of activator requirement are key advantages.
    • Understanding its principles and limitations is crucial for its effective application.