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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Apr 27, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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An ultraconserved lnc to miRNA processing.

Brian S Plosky1

  • 1Molecular Cell, Cell Press, 600 Technology Square, 5(th) Floor, Cambridge, MA 02139, USA.

Molecular Cell
|July 5, 2014
PubMed
Summary

Ultraconserved regions, previously uncharacterized, host a long non-coding RNA (lncRNA). This lncRNA was found to inhibit microRNA (miRNA) maturation, revealing a novel regulatory mechanism.

Area of Science:

  • Genomics and Molecular Biology
  • Non-coding RNA research
  • Gene regulation mechanisms

Background:

  • Ultraconserved regions (UCRs) are highly conserved DNA sequences across species.
  • Specific functions of UCRs remain largely unknown.
  • MicroRNAs (miRNAs) are key regulators of gene expression.

Purpose of the Study:

  • To investigate the functional role of a long non-coding RNA (lncRNA) transcribed from a UCR.
  • To elucidate the mechanism by which this lncRNA affects miRNA maturation.

Main Methods:

  • Identification and characterization of a novel lncRNA originating from a UCR.
  • Experimental assays to assess the impact of the lncRNA on miRNA processing and maturation.
  • Analysis of regulatory interactions between the lncRNA and miRNA biogenesis pathways.

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Main Results:

  • A lncRNA transcribed from a previously uncharacterized ultraconserved region was identified.
  • This lncRNA was demonstrated to negatively regulate the maturation of specific miRNAs.
  • The findings suggest a novel layer of gene regulation involving UCR-derived lncRNAs.

Conclusions:

  • Ultraconserved regions can serve as a source for functional regulatory RNAs.
  • The identified lncRNA plays a critical role in controlling miRNA maturation.
  • This study expands our understanding of gene regulation by non-coding RNAs and the functional significance of ultraconserved elements.