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Extraction: Advanced Methods

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...

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Related Experiment Video

Updated: Apr 27, 2026

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro

Published on: July 5, 2017

Polyphenol extraction optimisation from Ceylon gooseberry (Dovyalis hebecarpa) pulp.

Vivian Caetano Bochi¹, Milene Teixeira Barcia¹, Daniele Rodrigues¹

¹Department of Food Science, Faculty of Food Engineering, Campinas State University (UNICAMP), P.O. Box 6121, 13083-862 Campinas, SP, Brazil.

|July 6, 2014

Summary

This summary is machine-generated.

Optimized extraction of anthocyanins and total phenolics from Dovyalis hebecarpa berries was achieved using a simple, mild analytical technique. This method enhances yields, reduces time, and is less hazardous than traditional approaches.

Keywords:

Anthocyanin D. hebecarpa Extraction optimisation Phenolic Response surface methodology Simplex lattice design

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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Published on: June 10, 2022

Area of Science:

Food Science
Analytical Chemistry
Phytochemistry

Background:

Dovyalis hebecarpa, an exotic berry from Asia, is now cultivated in Brazil.
Limited literature exists on the phenolic extraction and characterization of this berry.

Purpose of the Study:

To optimize the extraction of anthocyanins and total phenolics from D. hebecarpa.
To develop a simple, mild, and efficient analytical technique for berry phytochemical analysis.

Main Methods:

Multivariate analysis was employed to optimize extraction variables: solvent proportions (ethanol:water:acetone), time, and acid concentration.
Optimized conditions involved acetone/water (20/80 v/v) or water-rich solvent mixtures, low formic acid concentration (0.35%), and minimal time (17.6 min).

Main Results:

The optimized method significantly increased anthocyanin (31.5%) and total phenolic (11%) extraction yields compared to traditional methods.
Acetone/water (20/80 v/v) and water-based solvent mixtures proved effective for anthocyanin extraction.
Extraction efficiency was largely unaffected by acid concentration and time, allowing for reduced levels.

Conclusions:

An optimized, simple, and mild analytical technique for extracting anthocyanins and total phenolics from D. hebecarpa was successfully developed.
The optimized method offers higher yields, is less time-consuming, and poses fewer hazards than conventional extraction techniques.
Freeze-dried D. hebecarpa exhibits substantial levels of anthocyanins (319 mg/100g) and total phenolics (1,421 mg/100g).