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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Three-dimensional polycaprolactone scaffold via needleless electrospinning promotes cell proliferation and
Dawei Li1, Tong Wu2, Nanfei He3
1Engineering Research Center of Technical Textiles, Donghua University, Shanghai 201620, China; College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Colloids and Surfaces. B, Biointerfaces
|July 6, 2014
Summary
Disc-electrospinning fabricates 3D scaffolds with enhanced protein adsorption and cell growth for tissue engineering. This porous polycaprolactone fiber scaffold shows improved cell attachment, proliferation, and migration, supporting soft tissue regeneration applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Electrospinning is common for tissue engineering scaffolds, but 2D membranes limit applications.
- Traditional needle-electrospinning (NE) has low production rates, hindering commercialization.
Purpose of the Study:
- To utilize disc-electrospinning (DE) for fabricating 3D porous scaffolds.
- To evaluate the potential of DE scaffolds for soft tissue regeneration.
Main Methods:
- Fabrication of 3D scaffolds using disc-electrospinning (DE) of polycaprolactone (PCL).
- Scanning electron microscopy (SEM) for morphology analysis.
- Protein adsorption assays and fibroblast cell culture for biological evaluation.
Main Results:
- DE PCL fibers exhibited a porous macro/nanoscale structure.
- Porous scaffolds showed 55% higher protein adsorption than solid fibers.
- Enhanced initial cell attachment, faster fibroblast proliferation, and cell migration up to 800μm into the scaffold were observed.
Conclusions:
- DE PCL fibers form effective 3D tissue engineering scaffolds.
- The porous structure enhances protein adsorption and cell interaction.
- DE offers a promising method for developing advanced scaffolds for soft tissue regeneration.
