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Updated: Apr 27, 2026
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
Real-time label-free measurement of HIV-1 protease activity by nanopore analysis
Liang Wang1, Yujing Han1, Shuo Zhou1
1Department of Biological and Chemical Sciences, Illinois Institute of Technology, Chicago, IL 60616, United States.
Abstract:
A label-free method for the measurement of the activity of HIV-1 protease is developed by real-time monitoring of the cleavage of a peptide substrate by HIV-1 protease in a nanopore. The method is rapid and sensitive: picomolar concentrations of HIV-1 protease could be detected in ~10 min. Simulated clinical samples are analyzed, and the activity of HIV-1 protease could be accurately detected. Our developed nanopore sensor design strategy should find useful applications in the development of stochastic sensors for other proteases of medical, pharmaceutical, and biological importance.
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