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Related Experiment Video

Updated: Apr 27, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
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Thromboelastography in healthy dairy cows.

Cora-Costanze Sommerey1, Tim L Williams1, Ian McCrone1

  • 1Department of Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.

Journal of Dairy Science
|July 7, 2014
PubMed
Summary
This summary is machine-generated.

Thromboelastography reference intervals were established for dairy cows. Coagulation parameters showed no significant changes across lactation periods or with sample storage up to 48 hours.

Keywords:
bovinecoagulationlactationreference intervalthromboelastography

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Area of Science:

  • Veterinary Medicine
  • Hemostasis and Thrombosis Research
  • Dairy Cattle Health

Background:

  • Coagulopathies are common in cows, necessitating reliable diagnostic tools.
  • Thromboelastography (TEG) assesses whole blood coagulation, offering insights into hypocoagulability and hypercoagulability.
  • Establishing species-specific reference intervals is crucial for accurate interpretation.

Purpose of the Study:

  • To determine reference intervals for TEG in healthy dairy cows using kaolin activation.
  • To investigate potential variations in TEG parameters based on lactation stage.
  • To assess the stability of citrated whole blood samples for TEG analysis over time.

Main Methods:

  • Sixty healthy Holstein-Friesian cows were grouped by lactation period (≤30, 31-99, ≥100 days postcalving).
  • Thromboelastography was performed using a TEG 5000 analyzer with citrated whole blood and kaolin activation.
  • Sample stability was tested for up to 100 hours at room temperature.

Main Results:

  • Reference intervals were established: reaction time (2.2–6.2 min), coagulation time (0.8–2.0 min), angle α (58.2–81.8°), maximum amplitude (64.3–89.2 mm), and clot rigidity (9.2–41.2 dyn/cm²).
  • No significant differences in TEG parameters were observed among the different lactation groups.
  • No significant changes in TEG parameters were detected in samples stored for up to 48 hours.

Conclusions:

  • The study provides essential reference intervals for TEG in dairy cows.
  • Lactation stage does not appear to significantly influence TEG parameters in healthy cows.
  • TEG samples are stable for at least 48 hours at room temperature, allowing for logistical flexibility.