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Published on: December 1, 2023
Cryopreservation of quince (Cydonia oblonga Mill.)
P T Lynch1, A Siddika2, A Mehra2
1Biological Sciences Research Group, University of Derby, Derby, UK. P.T.Lynch@derby.ac.uk.
Background:
Quince (Cydonia oblonga Mill.) has great potential for utilisation in pharmaceutical and food industries.
Objective:
The study was to develop an efficient cryopreservation approach for quince.
Methods:
Factors on the survival and regrowth such as cold acclimation, explant type and recovery media composition were assessed. The effectiveness of the resultant protocols for a number of quince cultivars was determined.
Results And Conclusion:
Quince shoot tips and nodal sections are successfully cryopreserved. Sustained regrowth of quince Angers A was observed after encapsulation-osmoprotection/dehydration, encapsulation-dehydration and PVS2 vitrification. The highest regrowth rate (80%) was obtained from explants excised from cold hardened shoots and cryopreserved using encapsulation-osmoprotection/dehydration and vitrification protocols. The optimised vitrification protocol in combination with shoot cold hardening and a MS recovery medium without activated charcoal and auxin resulted in satisfactory regrowth of shoots from six quince cultivars. The morphology of acclimatised plants derived from cryopreserved shoots was comparable with non-cryopreserved plants.
