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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution
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Engineering alginate as bioink for bioprinting.

Jia Jia1, Dylan J Richards1, Samuel Pollard1

  • 1Bioengineering Department, Clemson University, Clemson, SC 29634, USA.

Acta Biomaterialia
|July 8, 2014
PubMed
Summary

This study introduces oxidized alginate bioinks for 3-D bioprinting, enabling controlled degradation for advanced tissue engineering. These novel bioinks support human adipose-derived stem cell growth and spreading in printed structures.

Keywords:
Adipose-derived stem cellsBioinkBioprintingHydrogel scaffoldOxidized alginate

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Bioprinting Technology

Background:

  • Three-dimensional (3-D) printing offers solutions for tissue engineering challenges.
  • Alginate hydrogels are common bioinks, but native alginates have limited degradation.
  • Oxidized alginates with controlled degradation for bioprinting remain unexplored.

Purpose of the Study:

  • To develop an oxidized alginate-based bioink platform for diverse tissue engineering applications.
  • To investigate the impact of alginate oxidation and concentration on bioink properties and printability.
  • To assess the suitability of oxidized alginate bioinks for bioprinting human adipose-derived stem cells (hADSCs).

Main Methods:

  • Preparation of 30 alginate hydrogels with varying oxidation percentages and concentrations.
  • Systematic investigation of alginate solution viscosity and density for printability.
  • 3-D bioprinting of cell-laden lattice structures using four selected alginate bioinks and hADSCs.

Main Results:

  • Identified optimal ranges for alginate viscosity and density for successful bioprinting.
  • Achieved accurate printing of lattice-structured, cell-laden hydrogels with hADSCs.
  • Demonstrated that alginate bioinks modulated hADSC proliferation and spreading without compromising structural integrity (except highly degradable variants) after 8 days.

Conclusions:

  • Oxidized alginates provide a tunable bioink platform for 3-D bioprinting.
  • This approach supports cell viability and function within printed constructs.
  • Establishes a foundation for developing tissue-specific alginate-based bioinks for regenerative medicine.