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Updated: Apr 27, 2026

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Anti-Nuclear Antibody Screening Using HEp-2 Cells

Published on: June 23, 2014

Anti-nuclear antibody screening using HEp-2 cells.

Carol Buchner¹, Cassandra Bryant², Anna Eslami³

¹IFA Development Manager, INOVA Diagnostics, Inc.

Journal of Visualized Experiments : Jove

|July 8, 2014

Summary

The gold standard for antinuclear antibody (ANA) screening, indirect immunofluorescence (IIF), faces workflow challenges in modern labs. Digital imaging systems automate IIF, enhancing efficiency and accuracy for ANA testing.

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Area of Science:

Immunology
Laboratory Medicine

Background:

The American College of Rheumatology recommends indirect immunofluorescence (IIF) as the gold standard for antinuclear antibody (ANA) screening.
Traditional IIF methods present technical challenges, including labor-intensive slide processing, manual reading, and the need for specialized technologists and darkroom facilities, hindering integration into automated laboratory workflows.

Purpose of the Study:

To demonstrate the IIF procedure for ANA screening.
To introduce advancements in digital imaging systems designed to streamline and harmonize the IIF workflow.

Main Methods:

Detailed demonstration of IIF slide processing and identification of common IIF patterns.
Introduction of digital imaging systems, such as the NOVA View Automated Fluorescent Microscope, for automated slide reading and interpretation.

Main Results:

Digital imaging systems automate IIF by acquiring and storing high-resolution images, separating acquisition from interpretation.
These systems eliminate the need for darkrooms, streamline the reading/interpretation workflow, and improve consistency between readers.
Automated systems enhance patient data integrity and safety through sample traceability and elimination of transcription errors.

Conclusions:

Digital imaging systems offer a solution to the workflow limitations of traditional IIF for ANA screening.
These advancements improve efficiency, consistency, and patient safety in ANA testing.
Automated IIF reading enhances the reliability and accessibility of this crucial diagnostic technique.