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Increased soluble selectins as a reflection of activated platelets and endothelium in Legg-Calve-Perthes disease

Vusal Ismayilov1, Duygu Yazgan Aksoy, Nilgun Sayinalp

  • 1*Department of Orthopaedics and Traumatology ‡Section of Hematology, Department of Internal Medicine, Hacettepe University, Sihhiye, Ankara †Department of Internal Medicine, Acibadem University, Istanbul, Turkey.

Journal of Pediatric Hematology/Oncology
|July 8, 2014
PubMed

Insights

Elevated levels of E-selectin and P-selectin indicate endothelial and platelet activation in Legg-Calve-Perthes disease (LCPD). These findings suggest a role for microvascular thrombosis and inflammation in LCPD pathogenesis.

Area of Science:

  • Pediatric Orthopedics
  • Hematology
  • Vascular Biology

Background:

  • Legg-Calve-Perthes disease (LCPD) pathogenesis remains unclear, with prior studies suggesting hemostatic abnormalities.
  • Previous research indicated enhanced tissue factor pathway inhibitor response, increased global fibrinolytic capacity, and elevated thrombomodulin in LCPD patients, highlighting vascular endothelium involvement.

Purpose of the Study:

  • To investigate circulating E-selectin and P-selectin levels in pediatric patients diagnosed with Legg-Calve-Perthes disease.
  • To assess whether elevated levels of these markers reflect endothelial activation or injury in LCPD.

Main Methods:

  • The study included 85 pediatric patients, comprising 55 with LCPD (Group I) and 30 healthy children (Group II).
  • Peripheral venous blood samples were analyzed to measure E-selectin and P-selectin concentrations using a commercially available assay.

Main Results:

  • Both E-selectin and P-selectin levels were significantly higher in LCPD patients compared to age-matched controls.
  • Mean E-selectin levels were 54.92±18.84 pg/mL in LCPD patients versus 45.54±15.31 pg/mL in controls (P=0.022).
  • Mean P-selectin levels were 46.40±20.35 pg/mL in LCPD patients versus 36.92±9.84 pg/mL in controls (P=0.019).

Conclusions:

  • E-selectin and P-selectin, key markers of endothelial and platelet activation, are upregulated in Legg-Calve-Perthes disease.
  • The findings suggest that activated platelets and endothelial activation, indicated by P-selectin and E-selectin kinetics, may contribute to microvascular thrombosis and inflammation in LCPD.
Abstract

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