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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Apr 27, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Illuminating the future of DNA sequencing.

Mick Watson

    Genome Biology
    |July 9, 2014
    PubMed
    Summary

    Illumina

    Area of Science:

    • Genomics
    • Next-generation sequencing technologies

    Background:

    • The human genome sequencing market represents a significant commercial opportunity.
    • Sequencing companies are actively targeting this market.
    • Illumina's HiSeq X Ten platform has been announced with a stated limitation to human genome sequencing.

    Purpose of the Study:

    • To address concerns regarding the HiSeq X Ten platform's apparent limitation to human genome sequencing.
    • To clarify the technical capabilities versus licensing restrictions of the HiSeq X Ten.

    Main Methods:

    • Analysis of Illumina's platform specifications and licensing terms.
    • Review of presented PhiX control data from the HiSeq X Ten.

    Main Results:

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  • The HiSeq X Ten technology itself is not inherently limited to human genome sequencing.
  • The observed limitation is a result of Illumina's licensing strategy.
  • PhiX control data demonstrates the platform's capability beyond human applications.

    • Conclusions:

    • The restriction of the HiSeq X Ten to human genome sequencing is a business decision, not a technical one.
    • This licensing strategy is consistent with industry practices in the competitive human genome sequencing market.