Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geometry-dependent interfaces shape NLRP3 pyrin domain assembly.

International journal of biological macromolecules·2026
Same author

Investigating synergistic effects of key medium components and pectin elicitor on taxane fermentation in Neopestalotiopsis vitis: A Box-Behnken response surface methodology approach.

International microbiology : the official journal of the Spanish Society for Microbiology·2026
Same author

Inhibitory effect of silymarin on amyloid formation in ALS-associated hSOD1 P66R mutant.

International journal of biological macromolecules·2026
Same author

Design and fabrication of a platform for circulating tumor cells (CTCs) isolation and detection by MiRGD@NTA@Au-Fe<sub>3</sub>O<sub>4</sub> nanoparticles.

Biosensors & bioelectronics·2026
Same author

Suboptimal Responses to Anti-VEGF in Retinal Neurovascular Diseases: Linking Aging and Alternative Angioinflammatory Pathways.

Investigative ophthalmology & visual science·2026
Same author

Label-free naked-eye aptasensor for detection of Cytochrome c- mediated apoptosis drug screening.

Methods (San Diego, Calif.)·2026

Related Experiment Video

Updated: Apr 27, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

10.4K

Split-luciferase complementary assay: applications, recent developments, and future perspectives.

Taha Azad1, Amin Tashakor, Saman Hosseinkhani

  • 1Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Analytical and Bioanalytical Chemistry
|July 9, 2014
PubMed
Summary
This summary is machine-generated.

Split luciferase biosensors enable the study of protein-protein interactions and metabolite assays. These luminescent tools offer powerful applications in analytical biochemistry, cell biology, and diagnostics.

More Related Videos

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

23.4K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

18.9K

Related Experiment Videos

Last Updated: Apr 27, 2026

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

10.4K
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

23.4K
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

18.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Bioluminescent systems offer high quantum yields and broad color emission, making them potent reporter systems.
  • Protein-protein interactions are crucial for cellular functions and are mediated by large molecular complexes.
  • The split-luciferase complementation assay is a technique for studying protein interactions.

Purpose of the Study:

  • To review the applications of split luciferase biosensors in analytical biochemistry and molecular biology.
  • To highlight the utility of these biosensors in studying protein-protein interactions and metabolite assays.
  • To demonstrate the potential of luciferase-based biosensors for diagnostic and therapeutic purposes.

Main Methods:

  • Utilizing split luciferase complementation assay where luciferase fragments are fused to interacting proteins.
  • Inserting domains or intact proteins into internally fragmented luciferase for metabolite assays.
  • Analyzing luminescent signals generated by complemented luciferase or altered signals upon ligand binding.

Main Results:

  • Split luciferase effectively facilitates the study of protein-protein interactions.
  • The assay can be adapted for sensitive detection and quantification of biochemical metabolites.
  • Luminescent biosensors provide insights into cell communication, signaling, and cellular fate.

Conclusions:

  • Split luciferase-based biosensors are versatile tools for diverse biological and analytical applications.
  • These luminescent biosensors hold significant promise for diagnostic and therapeutic advancements.
  • The technology enables a deeper understanding of complex biological processes at the molecular level.