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Expression characteristics of two potential T cell mediator genes
B S Kwon1, D P Kestler, Z Eshhar
1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.
Cellular Immunology
|July 1, 1989
Summary
Two novel T cell mediators, 4-1BB and L2G25B, were identified. Their expression is inducible by T cell receptor stimulation but not by IL-2, suggesting a role in T cell responses.
Area of Science:
- Immunology
- Molecular Biology
Background:
- T lymphocyte subsets play crucial roles in immune responses.
- Novel mediators of T cell function are continuously being discovered.
Purpose of the Study:
- To characterize the expression patterns of two newly isolated T lymphocyte-specific cDNA clones, 4-1BB and L2G25B.
- To investigate the potential function of these molecules as T cell mediators.
Main Methods:
- Modified differential screening to isolate cDNA clones.
- Nucleotide sequence comparison.
- Analysis of transcript induction in various T cell populations using concanavalin A and T cell receptor (TCR) stimulation.
- Inhibition studies with cyclosporin A and IL-2 stimulation.
Main Results:
- 4-1BB represents a previously unrecognized cDNA.
- L2G25B sequence was recently identified, but its function is uncharacterized.
- Transcripts of both 4-1BB and L2G25B are inducible by concanavalin A and TCR stimulation in multiple T cell types.
- Inducibility is inhibited by cyclosporin A and not by IL-2.
- Expression patterns resemble IFN-gamma but with lower transcript levels and earlier peak expression.
Conclusions:
- 4-1BB and L2G25B may represent novel T cell mediators.
- Their induction patterns suggest a role in T cell activation and function.
- Further research is warranted to elucidate the precise functions of these molecules.