Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Larval fat body-specific gene expression in D. melanogaster.

J Deutsch1, M Laval, J A Lepesant

  • 1Institut Jacques Monod, CNRS Université Paris, France.

Developmental Genetics
|January 1, 1989
PubMed
Summary

Researchers mapped regulatory DNA sequences for the P1 gene in fruit flies. Key elements controlling fat body expression and ecdysone inducibility are located upstream of the gene.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of lingual oscillometric and invasive blood pressure measurement in dogs under general anaesthesia.

The Journal of small animal practice·2026
Same author

A quantitative evaluation of aerosol generation during cardiopulmonary resuscitation.

Anaesthesia·2023
Same author

A novel and effective ophthalmology morbidity and mortality meeting.

Eye (London, England)·2018
Same author

Faecal Escherichia coli as biological indicator of spatial interaction between domestic pigs and wild boar (Sus scrofa) in Corsica.

Transboundary and emerging diseases·2018
Same author

Institutional food as a lever for improving health in cities: the case of New York City.

Public health·2015
Same author

[Early childhood intervention - access to risk families and support through actors from the health-care sector].

Klinische Padiatrie·2014

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Drosophila melanogaster Research

Background:

  • The P1 gene is exclusively expressed in the larval fat body of Drosophila melanogaster during the third instar stage.
  • Understanding the regulation of P1 gene expression is crucial for studying developmental processes in fruit flies.

Purpose of the Study:

  • To identify and map the cis-acting regulatory sequences responsible for the temporal and spatial expression of the P1 gene.
  • To investigate the elements controlling fat body-specific transcription and ecdysone inducibility.

Main Methods:

  • Utilized hybrid constructs with three distinct reporter genes.
  • Employed a combination of in vivo transient and germline transformation assays for mapping regulatory sequences.
  • Performed in vivo mapping of cis-acting regulatory elements.

Related Experiment Videos

Main Results:

  • Identified a short DNA region immediately upstream of the P1 mRNA transcription start site containing critical regulatory elements.
  • Discovered an element within this region that functions as a fat-body transcriptional enhancer.
  • Found elements essential for the ecdysone inducibility of P1 gene transcription.

Conclusions:

  • The temporal and spatial specificity of P1 gene transcription is governed by regulatory elements located upstream of the gene.
  • These regulatory elements include a fat-body enhancer and elements mediating ecdysone responsiveness.
  • This study provides insights into the molecular mechanisms controlling gene expression in the Drosophila larval fat body.