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Published on: December 30, 2016
Purification, crystallization and preliminary X-ray diffraction analysis of Imp3 in complex with an Mpp10 peptide
1Department of Biochemistry and Molecular Biology, College of Life Sciences, Beijing Normal University, Beijing 100875, People's Republic of China.
Abstract:
Eukaryotic ribosome synthesis requires a vast number of transiently associated factors. Mpp10, Imp3 and Imp4 form a protein complex in the 90S pre-ribosomal particle that conducts early processing of 18S rRNA. Here, a short fragment of Mpp10 was identified to associate with and increase the solubility of Imp3. An Imp3-Mpp10 complex was co-expressed, co-purified and co-crystallized. Preliminary X-ray diffraction analysis revealed that the crystal diffracted to 2.1 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a=51.6, b=86.9, c=88.7 Å.
