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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos
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Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

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Ncor2 is required for hematopoietic stem cell emergence by inhibiting Fos signaling in zebrafish

Yonglong Wei1, Dongyuan Ma1, Ya Gao1

  • 1State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.

Blood
|July 10, 2014
PubMed

Insights

Nuclear receptor corepressor 2 (ncor2) is crucial for zebrafish hematopoietic stem cell (HSC) development. It represses fos-vegfd signaling, ensuring proper HSC emergence during embryonic development.

Area of Science:

  • Developmental Biology
  • Hematopoiesis
  • Molecular Biology

Background:

  • Nuclear receptor corepressors (Ncors) regulate vertebrate development and homeostasis.
  • The function of Ncors in definitive hematopoiesis remains largely unexplored.

Purpose of the Study:

  • To investigate the role of ncor2 in hematopoietic stem cell (HSC) development in zebrafish embryos.
  • To elucidate the molecular mechanisms by which ncor2 influences HSC emergence.

Main Methods:

  • Zebrafish embryo studies to examine ncor2 expression patterns.
  • Analysis of HSC populations in ncor2-deficient zebrafish.
  • Investigation of gene transcription and signaling pathways (fos, Vegfd, Notch) in ncor2 knockdown embryos.

Main Results:

  • ncor2 is specifically expressed in the aorta-gonad-mesonephros (AGM) region of zebrafish embryos.
  • ncor2 deficiency leads to reduced HSCs in the AGM region and decreased T cells in the thymus.
  • ncor2 knockdown upregulates fos transcription, enhances Vegfd signaling, and promotes arterial endothelial fate via Notch signaling, potentially inhibiting hemogenic endothelial specification.

Conclusions:

  • ncor2 plays a critical role in HSC development in zebrafish.
  • ncor2 acts by repressing the Fos-Vegfd-Notch signaling cascade, which is essential for HSC emergence.

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