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Related Experiment Video

Updated: Apr 27, 2026

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay

Published on: September 14, 2014

The HIV-1 Env trimer in HD.

James B Munro¹, Walther Mothes¹

¹Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, CT 06536, USA.

Structure (London, England : 1993)

|July 10, 2014

Summary

This summary is machine-generated.

Researchers used hydrogen-deuterium exchange (HDX) to reveal new details about the HIV-1 envelope (Env) trimer structure. This enhances understanding of how the HIV-1 Env protein activates for virus fusion, aiding vaccine and therapy development.

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Last Updated: Apr 27, 2026

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Published on: September 14, 2014

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

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Area of Science:

Structural Biology
Virology
Immunology

Background:

The native human immunodeficiency virus type 1 (HIV-1) envelope spike protein structure is crucial for developing effective vaccines and antiviral treatments.
Understanding the conformational dynamics of the HIV-1 Env trimer is key to deciphering its fusion mechanism.

Purpose of the Study:

To investigate the structural dynamics of the native HIV-1 Env trimer.
To gain new insights into the activation process of HIV-1 Env for virus fusion.

Main Methods:

Hydrogen-deuterium exchange (HDX) coupled with mass spectrometry was employed.
HDX-MS was used to probe structural changes and solvent accessibility of the HIV-1 Env trimer.

Main Results:

New structural insights into the HIV-1 Env trimer were obtained.
The study provides a better understanding of the conformational states involved in HIV-1 Env activation.

Conclusions:

HDX-MS is a powerful technique for studying the structural dynamics of the HIV-1 Env spike protein.
These findings contribute to the rational design of HIV-1 vaccines and therapeutics targeting Env structure and function.