Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Multi-Laboratory Assessment Reveals Variable Ion Species Profiles in Electrospray Ionization Mass Spectrometer".

Journal of the American Society for Mass Spectrometry·2026
Same author

Untargeted metabolomics of mature sorghum [<i>Sorghum bicolor</i> (L.) Moench] grain reveals metabolites associated with antimicrobial activity against <i>Clostridium perfringens</i>.

Frontiers in plant science·2026
Same author

Multi-Laboratory Assessment Reveals Variable Ion Species Profiles in Electrospray Ionization Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

Best Practices in GC-MS and GC × GC-MS-Based Metabolomics and Volatile Analyses: An International Survey.

Analytical chemistry·2026
Same author

Comparison of Extraction Methods for the Quantification of Phytohormones from Tomato Fruits and Leaves by LC-MS/MS.

bioRxiv : the preprint server for biology·2026
Same author

A sorghum pangenome reference improves global crop trait discovery.

Nature·2026
Same journal

PGPR-AMF consortia improve drought tolerance in maize through stomatal regulation, antioxidant defense, and yield stability.

Frontiers in plant science·2026
Same journal

Correction: The 1-aminocyclopropane-1-carboxylic acid deaminase-producing <i>Streptomyces violaceoruber</i> UAE1 can provide protection from sudden decline syndrome on date palm.

Frontiers in plant science·2026
Same journal

An integrated edge AI prototype for smart agriculture: real-time pest detection, physical trapping, and multi-node deployment analysis under field uncertainty.

Frontiers in plant science·2026
Same journal

Twice-spraying plant growth regulator EDAH stage-regulates maize plant morphology: a novel strategy for enhancing stalk lodging resistance.

Frontiers in plant science·2026
Same journal

A FnWRKY17-<i>FnFLA16</i> regulatory module controls leaf curling in <i>Fragaria nilgerrensis</i>.

Frontiers in plant science·2026
Same journal

24-epibrassinolide-mediated regulation of physiological and biochemical parameters in <i>Chlamydomonas reinhardtii</i> for optimized growth and future biotechnological applications.

Frontiers in plant science·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
07:10

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

8.7K

Evaluating plant immunity using mass spectrometry-based metabolomics workflows.

Adam L Heuberger1, Faith M Robison1, Sarah Marie A Lyons2

  • 1Proteomics and Metabolomics Facility, Colorado State University Fort Collins, CO, USA ; Department of Soil and Crop Sciences, Colorado State University Fort Collins, CO, USA.

Frontiers in Plant Science
|July 11, 2014
PubMed
Summary
This summary is machine-generated.

Plant metabolomics studies reveal how metabolic processes contribute to disease resistance. Optimizing metabolomics workflows, especially mass spectrometry data analysis, is crucial for understanding plant immunity and defense responses across different plant-pathogen systems.

Keywords:
GC-MSLC-MSmetabolomicsplant defenseplant pathogen

More Related Videos

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
05:51

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

649
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

8.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
07:10

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

8.7K
Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format
05:51

Evaluating Leaf Responses to Microbial Secondary Metabolites Using A High-Throughput Format

Published on: December 5, 2025

649
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
06:43

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Published on: June 21, 2018

8.3K

Area of Science:

  • Plant Science
  • Biochemistry
  • Molecular Biology

Background:

  • Metabolic processes are integral to plant physiological and biochemical disease resistance.
  • Metabolomics offers a systems-level view of plant metabolism during defense responses.
  • Plant immunity research relies heavily on metabolomics to understand complex biochemical interactions.

Purpose of the Study:

  • To review current metabolomics workflows used in plant defense studies.
  • To highlight the impact of workflow choices on interpreting plant immunity data.
  • To identify areas for improvement in metabolomics data analysis and reporting for plant-pathogen systems.

Main Methods:

  • Literature review of existing metabolomics workflows in plant science.
  • Analysis of detection, annotation, and interpretation methods in plant metabolomics.
  • Examination of mass spectrometry (MS) data analysis techniques.

Main Results:

  • A wide variety of metabolomics workflows are currently employed in plant research.
  • The selection of a metabolomics workflow significantly influences the conclusions drawn about plant defense.
  • Multiple chemical detection platforms and non-targeted approaches are often necessary due to the complexity of plant defense metabolites.

Conclusions:

  • Standardizing and improving metabolomics workflows can enhance the comparability of findings across plant-pathogen studies.
  • Advancements in mass spectrometry data analysis are key to translating metabolomics insights.
  • Optimized metabolomics approaches are essential for a comprehensive understanding of plant immunity.