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A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
11:15

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

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miR-155 mediates drug resistance in osteosarcoma cells via inducing autophagy

Lu Chen1, Ke Jiang1, Hua Jiang2

  • 1Orthopedics Department, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China.

Experimental and Therapeutic Medicine
|July 11, 2014
PubMed

Insights

MicroRNA-155 (miR-155) induces autophagy in osteosarcoma cells, leading to chemotherapy resistance. This study reveals a novel mechanism where miR-155 promotes drug resistance by upregulating autophagy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Drug resistance is a major challenge in osteosarcoma chemotherapy.
  • Autophagy plays a significant role in osteosarcoma development and therapy resistance.
  • The precise mechanisms of autophagy-mediated chemoresistance in osteosarcoma are not fully understood.

Purpose of the Study:

  • To investigate the role of microRNA-155 (miR-155) in chemotherapy-induced autophagy in osteosarcoma cells.
  • To elucidate the molecular mechanisms by which miR-155 influences chemoresistance in osteosarcoma.

Main Methods:

  • Measuring autophagy and miR-155 expression in osteosarcoma cells treated with chemotherapeutic drugs.
  • Assessing the effect of miR-155 on chemotherapy-induced autophagy and cell viability.

Main Results:

  • Chemotherapy significantly induced both autophagy and miR-155 expression in osteosarcoma cells.
  • Increased miR-155 levels enhanced chemotherapy-induced autophagy.
  • Upregulated miR-155 expression ameliorated the decrease in cell proliferation and viability caused by anticancer drugs.

Conclusions:

  • MicroRNA-155 mediates drug resistance in osteosarcoma by inducing autophagy.
  • This study identifies a novel mechanism contributing to chemoresistance in osteosarcoma.
Keywords:
autophagydrug resistancemicroRNA-155osteosarcoma cells

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