Volume Holographic Reflection Endoscope for In-Vivo Ovarian Cancer Clinical Studies.

Area of Science:

• Biomedical Optics
• Medical Imaging
• Endoscopy

Background:

• Ovarian tissue imaging requires high resolution and subsurface visualization.
• Current endoscopic methods often lack simultaneous multi-depth imaging capabilities.
• Holographic techniques offer potential for advanced optical sectioning.

Purpose of the Study:

• To design and demonstrate the first endoscopic volume holographic imaging system.
• To achieve simultaneous imaging of ovarian tissue at two distinct depths.
• To evaluate the system's resolution and performance for potential clinical applications.

Main Methods:

• A multiplexed volume hologram was employed to select wavefronts from different tissue depths.
• The endoscope integrated gradient index (GRIN) and singlet lenses for image relay.
• The system utilized a 3.75 mm diameter endoscope with holographic components in the handle.
• Phenanthraquinone-doped poly methacrylate was used to record the volume hologram.

Main Results:

• The system achieved simultaneous imaging at the tissue surface and 70 μm below.
• Lateral resolution was measured at approximately 4 μm at a 660 nm wavelength.
• The method requires no mechanical scanning and operates at the camera frame rate.
• Preliminary ex-vivo results showed good correlation with histology.

Conclusions:

• The developed endoscopic volume holographic system enables simultaneous multi-depth imaging of ovarian tissue.
• The system demonstrates high lateral resolution and compatibility with standard histology.
• This technology offers a promising non-mechanical approach for subsurface tissue visualization.