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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
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N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1

Yuki Goto1, Yuki Niwa1, Takehiro Suzuki2

  • 1Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan.

FEBS Open Bio
|July 11, 2014
PubMed

Keywords:
CBB, Coomassie Brilliant BlueDMEM, Dulbecco’s modified Eagle’s mediumECM, extracellular matrixER, endoplasmic reticulumEnzymatic activityHA, hyaluronic acidHYAL1, hyaluronidase1Hyaluronidase1MALDI-TOF MS, matrix-assisted laser desorption ionization time of flight mass spectrometryN-glycosylationPBS, phosphate-buffered salineSDS, sodium dodecyl sulfateSecretion

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