Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antifungal Agents01:15

Antifungal Agents

114
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to...
114
Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

2.1K
Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut Microbiota Drives Aging-related Erythropoiesis Impairment via Phenylacetic Acid-induced Histone Phenylacetylation.

Blood·2026
Same author

A coumarin-based water-soluble fluorescent probe for tandem detection of Cu<sup>2+</sup> and glutathione with application in bioimaging and real sample analysis.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Naringenin inhibits endothelial-mesenchymal transition and alleviates myocardial fibrosis in rats by regulating the AKT/GSK3β/β-catenin pathway.

Molecular and cellular biochemistry·2026
Same author

Rosmarinic acid ameliorates radiation-induced intestinal injury through PI3K/AKT/NF-κB/MMP9 axis inhibition: From network pharmacology prediction to experimental validation.

Tissue & cell·2026
Same author

Time-programmed Losmapimod release enhances ANXA1-associated efferocytosis for diabetic wound repair.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Robust self-assembled monolayer enables ultraviolet stable perovskite photovoltaics.

Nature communications·2026

Related Experiment Video

Updated: Apr 27, 2026

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
15:04

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Published on: September 28, 2019

5.3K

Molecular interactions between amantadine and model cell membranes.

Fu-Gen Wu1, Pei Yang, Chi Zhang

  • 1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, China.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 11, 2014
PubMed
Summary

Sum frequency generation (SFG) spectroscopy revealed how amantadine interacts with model cell membranes. Amantadine affects lipid packing and flip-flop rates differently depending on lipid charge and drug concentration, offering insights into drug-membrane mechanisms.

More Related Videos

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.2K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.3K

Related Experiment Videos

Last Updated: Apr 27, 2026

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
15:04

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Published on: September 28, 2019

5.3K
Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
11:44

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Published on: January 19, 2022

2.2K
Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.3K

Area of Science:

  • Biophysics
  • Chemical Physics
  • Materials Science

Background:

  • Understanding drug-membrane interactions is crucial for drug development and delivery.
  • Lipid bilayers serve as essential models for cell membranes, enabling the study of molecular mechanisms.
  • Sum frequency generation (SFG) vibrational spectroscopy is a powerful surface-sensitive technique for probing molecular structures and dynamics at interfaces.

Purpose of the Study:

  • To investigate the molecular interactions between the water-soluble drug amantadine and model cell membranes (lipid bilayers).
  • To elucidate how amantadine influences the packing state and flip-flop process of lipid bilayers.
  • To determine the drug-membrane interaction mechanism at a molecular level.

Main Methods:

  • Sum frequency generation (SFG) vibrational spectroscopy was employed.
  • Studies utilized both isotopically asymmetric and symmetric lipid bilayers (PC and DPPG).
  • Deuterated lipid bilayers were used to examine amantadine distribution and orientation.

Main Results:

  • Amantadine associates with zwitterionic PC bilayers with minimal effect on flip-flop behavior at low concentrations.
  • Amantadine significantly impacts negatively charged DPPG bilayers, disturbing outer leaflet packing.
  • Amantadine binds to the outer leaflet of DPPG bilayers and does not translocate to the inner layer, with concentration-dependent effects on lipid order.

Conclusions:

  • SFG spectroscopy provides detailed molecular-level insights into drug-membrane interactions, surpassing other techniques.
  • Amantadine's interaction with lipid bilayers is dependent on lipid charge and drug concentration.
  • The findings have implications for amantadine's use in liposomal drug delivery systems and understanding its membrane interaction mechanisms.