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Updated: Apr 27, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
Published on: April 13, 2016
Metalloproteomics: focus on metabolic issues relating to metals
Eve A Roberts1, Bibudhendra Sarkar
1aDivision of Gastroenterology, Hepatology and Nutrition, The Hospital for Sick Children bGenetics and Genome Biology Program cMolecular Structure and Function Program, The Hospital for Sick Children Research Institute dDepartments of Paediatrics eMedicine fPharmacology gBiochemistry, University of Toronto, Toronto, Ontario, Canada.
Metalloproteomics, the study of metal-binding proteins, has advanced significantly. This field now investigates various metals and microbial systems, offering insights into metabolism and potential biomarkers.
Area of Science:
- Biochemistry
- Metabolomics
- Nutritional Science
Background:
- Metalloproteomics utilizes high-throughput methods to study metal-related nutritional and metabolic processes.
- The field has evolved over approximately 12 years, initially focusing on copper in relation to Wilson disease.
Purpose of the Study:
- To review recent advancements in metalloproteomics.
- To highlight its application in understanding metal roles in nutrition and metabolism.
Main Methods:
- High-throughput methodology for determining metalloproteomes.
- Analysis of metalloproteomes for single or multiple metals within defined systems.
Main Results:
- Recent research has expanded beyond copper to include nickel, zinc, and manganese metalloproteomes.
- Microbial metalloproteomics is emerging as a significant area of study due to the microbiome's role in metabolism.
Conclusions:
- Metalloproteomics is a key research strategy for investigating metal roles in metabolic physiology across diverse organisms.
- Technological progress has made metalloproteomics an innovative tool for understanding human metabolism, potentially identifying diagnostic biomarkers.
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