[Soy lipoxygenase as the object for the primary testing of potential inhibitors of leukotriene biosynthesis]

The comparative study of the ability of three compounds (indomethacin, agent BW 755 C and arachidonate hydroxamic acid) to inhibit the activity of preparations of soya bean lipoxygenase and to block leukotriene C4 biosynthesis in human leukocytes recorded by reversed phase liquid chromatography was performed. It was shown that irrespectively of purification degree soya bean lipoxygenase is a sufficiently adequate object for primary testing of inhibitors of biosynthesis of leukotrienes with the given type of action. Compounds with IC50 less than or equal to 1.10(-6) mol/l might be considered promising for the further investigation.