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Related Concept Videos

Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

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Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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Related Experiment Video

Updated: Apr 27, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
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[SSR information in Erigeron breviscapus transcriptome and polymorphism analysis].

Yin Chen, Cui-Ting Li, Ni-Hao Jiang

    Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica
    |July 12, 2014
    PubMed
    Summary

    This study identified numerous simple sequence repeats (SSRs) in the Erigeron breviscapus transcriptome, providing valuable molecular markers for genetic diversity and mapping. The findings lay the groundwork for developing new functional gene SSR markers.

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    Area of Science:

    • Plant genomics
    • Molecular biology
    • Bioinformatics

    Context:

    • The Erigeron breviscapus transcriptome was analyzed to identify simple sequence repeats (SSRs).
    • SSR markers are valuable tools for genetic studies in plants.
    • Understanding SSR distribution is crucial for marker development.

    Purpose:

    • To analyze SSR information within the Erigeron breviscapus transcriptome.
    • To lay the foundation for developing novel functional gene SSR markers.
    • To assess the potential of identified SSRs for genetic applications.

    Summary:

    • A total of 3639 SSRs were identified in 3260 unigenes from the Erigeron breviscapus transcriptome.
    • Di-nucleotide repeats were the most frequent type (34.41%), with AT/AT and AC/GT motifs being predominant.
    • Primer validation showed high amplification success (94.44%) and significant polymorphism (52.78%), dividing plants into two groups.

    Impact:

    • Identified numerous SSRs in Erigeron breviscapus, offering abundant candidate molecular markers.
    • Facilitates future genetic diversity studies and the construction of genetic maps for this species.
    • Provides essential genomic resources for breeding programs and evolutionary research.