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Related Experiment Videos

Lymphocyte subset counting by immunoperoxidase using an automated routine hematologic analyzer.

G d'Onofrio, G Menichella, G Zini

    Haematologica
    |March 1, 1989
    PubMed
    Summary

    The Technicon H*1 analyzer accurately counts lymphocyte subsets using immunoperoxidase methods. This automated technique shows good precision and clinical utility for assessing immune status, particularly in HIV infection.

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    Area of Science:

    • Immunology
    • Hematology
    • Clinical Diagnostics

    Background:

    • Accurate lymphocyte subset enumeration is crucial for immune status assessment.
    • Existing methods like manual immunofluorescence can be imprecise.
    • Automated hematology analyzers offer potential for improved efficiency and accuracy.

    Purpose of the Study:

    • To evaluate the technical performance and clinical usefulness of the Technicon H*1 analyzer for lymphocyte subset counting.
    • To compare the H*1 method with established techniques like flow cytometry and manual microscopy.
    • To establish reference ranges and assess clinical applicability in HIV-infected individuals.

    Main Methods:

    • Utilized monoclonal antibodies against CD2, CD4, CD8, and CD19 antigens.
    • Employed an automated biotin-avidin immunoperoxidase technique on whole blood samples.

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  • Compared results with immunofluorimetric flow cytometry (Ortho Spectrum III) and manual immunofluorescence.

    • Main Results:

    • The Technicon H*1 method demonstrated excellent precision and strong correlation with the Ortho Spectrum III flow cytometer.
    • Correlation with manual immunofluorescence was significantly poorer due to manual method imprecision.
    • Established reference ranges using the H*1 method closely matched those from the Spectrum III.
    • Observed significant decreases in CD4+ cells and the CD4+/CD8+ ratio in HIV-infected subjects, correlating with disease progression.

    Conclusions:

    • The Technicon H*1 analyzer provides a precise and clinically useful method for lymphocyte subset enumeration.
    • This automated approach offers advantages over less precise manual techniques.
    • The H*1 method is valuable for monitoring immune status in patients with or at risk for HIV infection.