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Updated: Apr 27, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay mPCR/RLB
Published on: August 6, 2011
Simple multiplex real-time PCR for rapid detection of common 16S rRNA methyltransferase genes
Xuejun Guo1, Belinda B Dillon2, Andrew N Ginn2
1Centre for Infectious Diseases and Microbiology, University of Sydney, Westmead Hospital, Sydney, New South Wales, Australia.
Abstract:
We have developed a real-time multiplex PCR assay to detect the three most common 16S rRNA methyltransferase genes (armA, rmtB and rmtC), which encode problematic high-level resistance to all clinically-relevant aminoglycoside antibiotics. All results were consistent with published conventional PCR assays and these genes still appear rare in Australia.
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