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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Stem Cell Therapy for Tissue Regeneration01:21

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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
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Bone Marrow Sampling and Transplants01:22

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Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
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Related Experiment Video

Updated: Apr 27, 2026

Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
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Human Bone Marrow-derived Mesenchymal Stem Cell: A Source for Cell-Based Therapy.

M Ayatollahi1, B Geramizadeh1, M Zakerinia2

  • 1Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

International Journal of Organ Transplantation Medicine
|July 12, 2014
PubMed
Summary
This summary is machine-generated.

Mesenchymal stem cells (MSCs) were successfully isolated and cultured from human bone marrow. These MSCs demonstrated key immunophenotypic markers and differentiation potential, confirming their suitability for cell-based therapies.

Keywords:
Human bone marrowMesenchymal stem cellscell therapy

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Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Immunology

Background:

  • Mesenchymal stem cells (MSCs) possess multipotency and immunomodulatory properties.
  • These characteristics make MSCs promising for cell transplantation and tissue engineering applications.

Purpose of the Study:

  • To isolate, culture, and characterize human bone marrow-derived MSCs.
  • To confirm MSC identity using immunophenotypic markers and assess their differentiation capacity.

Main Methods:

  • Bone marrow mononuclear cells were isolated using density-gradient centrifugation.
  • Adherent cells were cultured and expanded, with immunophenotypic analysis by flow cytometry.
  • In vitro differentiation into osteoblasts and adipocytes was performed.

Main Results:

  • Isolated MSCs expressed high levels of CD90 and CD73.
  • MSCs were negative for hematopoietic and endothelial markers (CD45, CD34, CD31).
  • Successful osteogenic and adipogenic differentiation was confirmed via Alizarin Red and Oil Red staining, respectively.

Conclusions:

  • Human bone marrow is a viable source for MSC isolation and expansion.
  • Characterized MSCs are suitable for potential cell-based therapeutic applications.