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Updated: Apr 27, 2026
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Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
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Notch signaling functions in retinal pericyte survival
Joseph F Arboleda-Velasquez1, Vincent Primo1, Mark Graham2
1Schepens Eye Research Institute, Mass Eye and Ear Infirmary and Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
Investigative Ophthalmology & Visual Science
|July 13, 2014
Summary
Notch signaling is crucial for retinal pericyte survival, protecting them from cell death. This study identifies key genes regulated by Notch, offering insights into diabetic retinopathy and vascular stability.
Area of Science:
- Vascular biology
- Cell signaling
- Ophthalmology
Background:
- Pericytes are vital for vascular development and stability.
- Pericyte loss contributes to diseases like diabetic retinopathy.
- Pathways regulating retinal pericyte survival are poorly understood.
Purpose of the Study:
- Investigate the role of Notch signaling in retinal pericyte survival.
- Identify Notch-regulated genes involved in pericyte survival.
Main Methods:
- Developed a coculture system for ligand-dependent Notch signaling.
- Used quantitative PCR and cell death assays to assess Notch activity.
- Analyzed Notch gain- and loss-of-function effects on apoptosis-related proteins.
Main Results:
- Retinal pericytes express key Notch pathway molecules.
- Notch signaling activates canonical Notch/RBPJK targets.
- Ligand-mediated Notch activity protected pericytes from cell death.
- Identified survival genes downstream of Notch in pericytes.
Conclusions:
- Notch signaling plays a critical role in retinal pericyte survival.
- Identified signature genes regulated by Notch in pericytes.
- Tight regulation of Notch signaling is essential for maintaining pericyte health.
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