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Progesterone receptor B promoter hypermethylation in human placenta after labor onset

Yanyan Zhuang1, Hong Cui1, Sishi Liu1

  • 1Division of Gynecology and Obstetrics, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China.

Reproductive Sciences (Thousand Oaks, Calif.)
|July 13, 2014
PubMed

To determine the methylation status of progesterone receptor B (PR-B) promoter and how PR-B regulates progesterone action in placenta during human pregnancy. Placentas were obtained from the pregnancy women at term who underwent cesarean delivery and vaginal delivery. The methylation status of the PR-B promoter was analyzed using the methylation-specific polymerase chain reaction (PCR) and bisulfite sequencing PCR. And the messenger RNA (mRNA) and protein expression of the PR-B and DNA methyltransferases (DNMTs) were determined by quantitative real-time PCR and Western blot. Compared with the cesarean group, the placentas of vaginal delivery group had greater levels of PR-B DNA methylation, and the PR-B, DNMT1, DNMT3a, and DNMT3b mRNA and protein expression were significantly decreased. Progesterone receptor B methylation occurs with high frequency after labor onset and may play an important epigenetic mechanism of labor-related PR-B negative expression, thereby mediating the biological process of functional progesterone withdrawal at term for parturition.

Keywords:
DNA methylationDNA methyltransferaseshuman parturitionplacentaprogesterone receptor B

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