History of Pu'er Tea and comparative study for the effect of its various extracts on lipid-lowering diet

Pu'er Tea is a kind of traditional historical famous tea which gains its name for native government jurisdiction in Pu'er (now Xishuangbanna in Yunnan, Pu'er city etc), and takes Pu'er (now Ninger county of Pu'er city) city as its collecting and distributing center .It is famous all over the world because of its good benefits for reducing blood lipid, slimming weight, antibacterial, aid digestion, detoxification and other functions, it is even known as the health care beverage with "the fine quality goods for preserving people's health", "a health drink demanded everyday". Although there are a lot of current study literature about the effect of Pu'er Tea on lipid-lowering and reducing weight, but there is rarely contrast study about the effect of lipid-lowering diet with its various extracts. Therefore, this article uses the acetone, water, chloroform, ethyl acetate and n-butanol to continuously extract Pu'er Tea, then freeze and dry them into four major separate components which include the chloroform layer, ethyl acetate layer, butanol layer and the remaining water layer. Take advantage of different components for filling and feeding the ICR mice which are treated with the processing of obesity molding, then compare the extract of Pu'er Tea with the weight-loss drug L-carnitine which is popular all over the market, explore the slimming effect of each component in Pu'er Tea on the cells of ICR fat mice. The results show that the total water extract of Pu'er Tea, ethyl acetate extract, residual water extract all have obvious effect on reducing body weight and body fat of experimental mice, it also has significant lowering effect on blood lipid and liver lipid in mice, that could significantly inhibit the accumulation of lipid in fat cells and hypertrophy of fat cells, reveal that the Pu'er Tea has good function of lipid-lowering and reducing weight. At the same time, the comprehensive effect of lipid-lowering and reducing weight through Pu 'er Tea is superior to commercial weight loss drug L-carnitine, and weight reducing effect of ethyl acetate extracts in Pu'er Tea and residual water layer is better than total water extract of Pu'er Tea. But n-butyl extract doesn't show a significant effect of lipid-lowering and reducing weight, inferring that efficacy component of Pu'er Tea which plays a major role of the effective component of lipid-lowering and reducing weight may exist in ethyl acetate extract and residual layer material.