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The 5S RNA gene minichromosome of Euplotes
A E Roberson1, A P Wolffe, L J Hauser
1U.T.-Oak Ridge Graduate School of Biomedical Sciences, Biology Division, TN 37831-8077.
Nucleic Acids Research
|June 26, 1989
Abstract:
The macronucleus of the ciliated protozoan Euplotes eurystomus contains about 10(6) copies of a single type of 5S ribosomal RNA gene. This 5S gene DNA is only 930 bp long, is flanked by telomeres, and contains a single coding region of 120 bp which serves as a template for transcription in vivo and in vitro. The 5S gene minichromatin possesses four positioned nucleosomes and hypersensitive cleavage sites in the telomeric regions.