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Updated: Apr 27, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?
Hanne C Thomsen1, Dennis Eriksson1, Inge S Møller1
1Department of Plant and Environmental Sciences, Plant and Soil Science Section, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark.
Abstract:
Overexpression of the cytosolic enzyme glutamine synthetase 1 (GS1) has been investigated in numerous cases with the goal of improving crop nitrogen use efficiency. However, the outcome has generally been inconsistent. Here, we review possible reasons underlying the lack of success and conclude that GS1 activity may be downregulated via a chain of processes elicited by metabolic imbalances and environmental constraints. We suggest that a pivotal role of GS1 may be related to the maintenance of essential nitrogen (N) flows and internal N sensing during critical stages of plant development. A number of more refined overexpression strategies exploiting gene stacking combined with tissue and cell specific targeting to overcome metabolic bottlenecks are considered along with their potential in relation to new N management strategies.
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