Related Experiment Video

Updated: Aug 16, 2026

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
15:57

Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

Published on: July 11, 2015

[An immunologic method for identification of M. tuberculosis and M. bovis (BCG) based on the use of monoclonal

M V Androsova, M A Vladimirskiĭ, G I Alekseeva

    Problemy Tuberkuleza
    |January 1, 1989
    PubMed

    Monoclonal antibodies interacting with specific selectivity with human tubercle bacilli (monoclonal antibodies of the cell producing strain 62D) and bovine tubercle bacilli (BCG) (monoclonal antibodies of the cell producing strain 60D) were produced with hybridoma technology. A rapid, productive and safe procedure for identification of M. tuberculosis and M. bovis (BCG) was developed on the basis of solid phase enzyme immunoassay. The immunological procedure was compared with the biochemical (niacin) method for identification of mycobacteria. The immunological procedure was shown to be reliable and promising for practical use.

    More Related Videos

    Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System10:11

    Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System

    Published on: January 31, 2018

    Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis06:26

    Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis

    Published on: July 28, 2023

    Related Concept Videos

    Hybridoma Technology01:31

    Hybridoma Technology

    Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
    Hybridoma Selection
    Commonly used fusion techniques — electroporation, polyethylene glycol...
    Pulmonary Tuberculosis IV01:26

    Pulmonary Tuberculosis IV

    Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
    Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...