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Updated: Apr 27, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
TGF β1 mediates epithelial mesenchymal transition via β6 integrin signaling pathway in breast cancer
Wentong Li1, Baogang Zhang, Hongli Li
1Department of Pathology, Weifang Medical University , Weifang, Shandong Province , P.R. China , 1.
Background:
To understand the function of β6 integrin and elucidate its signaling pathways in TGF-β-induced EMT in breast cancer.
Methods:
The interactions between TGF-β1 and β6 integrin were measured by coimmunoprecipitation. The EMT responses, phospherlation of PI3K/Akt and COX-2 expression were determined by real-time PCR, transwell assay, and western blot after the blockage of β6 integrin.
Results:
TGF-β1 and β6 integrin could bind with each other. Blockage of β6 integrin rescued TGF-β1-induced EMT phenotype and reduced expression of COX-2 via dephosphorylation of PI3K/Akt.
Conclusions:
β6 integrin plays a critical role in TGF-β1-induced EMT and overexpression of COX-2 in breast cancer.
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