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Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites
Published on: February 14, 2020
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A novel, simplified technique to amplify Eimeria (Coccidia: Apicomplexa) DNA from oocysts
R W Gerhold1, L R McDougald, R B Beckstead
1Department of Poultry Science, University of Georgia, Athens, Georgia 30602;
The Journal of Parasitology
|July 15, 2014
Abstract:
A new method to amplify coccidia DNA by polymerase chain reaction (PCR) was developed by placing freeze-thawed oocysts in Ready-to-Go PCR bead tubes and using a 5-min initial heat denaturation step. Positive PCR reactions were found in 3 of 3 samples containing 20 or 50 oocysts; when ≤5 oocysts were used, 1 of 3 samples was positive. This technique shows potential for effectively and efficiently detecting and identifying oocysts from soil, feces, and other matter.

