Related Experiment Video
Updated: Apr 27, 2026

Accessing Valuable Ligand Supports for Transition Metals: A Modified, Intermediate Scale Preparation of 1,2,3,4,5-Pentamethylcyclopentadiene
Published on: March 20, 2017
Stepwise elusion method in micellar electrokinetic chromatography via sequential use of lithium perfluorooctadecyl
Yukihiro Esaka1, Fumiaki Rin2, Miki Kobayashi2
1Gifu Pharmaceutical University, Gifu 501-1196, Japan; United Graduate School of Drug Discovery and Medical Information Science, Gifu University, Gifu 501-1193, Japan.
Abstract:
An effective stepwise micellar electrokinetic chromatography (MEKC) elution method was developed using lithium perfluorooctadecyl sulfonate (LPFOS) and lithium dodecyl sulfate (LDS). The hydrogen-bonding property of LPFOS micelles differs from that of LDS micelles, which leads to remarkably different selectivity in the transfer of solutes to the micelles. The present stepwise method is performed by replacing the inlet reservoir of a first running solution containing LPFOS with that of a second running solution containing LDS during a single separation run in the absence of electroosmotic flow under acidic conditions, where LPFOS micelles work as carriers in first and then LDS micelles turn over. Effective separation of 15 nonionic aromatic compounds was controlled well by adjusting the time in the inlet reservoir, which could not be accomplished with systems using only LPFOS or only LDS, with significant changes in the elution order where necessary. Furthermore, separations with the present stepwise method were easily simulated, and the replacement time was optimized for 3.1min from a 70.0mM LPFOS solution to a 67.5mM LDS solution with nearly complete separation within 15min using the simulated parameters.
More Related Videos
08:40Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
09:54Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
Published on: November 19, 2015
Related Concept Videos
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
High-Performance Liquid Chromatography: Elution Process
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...
Ion-Exchange Chromatography
Electrophoresis: Overview
There...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...