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Updated: Apr 27, 2026

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

Published on: September 9, 2015

Revised reference broth microdilution method for testing telavancin: effect on MIC results and correlation with other

David J Farrell¹, Rodrigo E Mendes², Paul R Rhomberg²

¹JMI Laboratories, North Liberty, Iowa, USA david-farrell@jmilabs.com.

Antimicrobial Agents and Chemotherapy

|July 16, 2014

Summary

This summary is machine-generated.

A revised broth microdilution method for telavancin, using dimethyl sulfoxide (DMSO) and polysorbate 80 (P-80), provides more accurate antimicrobial susceptibility testing results. This updated method ensures proper telavancin concentration, leading to lower MIC values, especially for staphylococci and enterococci.

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Last Updated: Apr 27, 2026

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Area of Science:

Microbiology
Pharmacology
Clinical Laboratory Science

Background:

The reference broth microdilution (BMD) method for telavancin required revision for water-insoluble agents.
Dimethyl sulfoxide (DMSO) and polysorbate 80 (P-80) were incorporated to address solubility and binding issues.

Purpose of the Study:

To evaluate a revised BMD method for telavancin susceptibility testing.
To compare results with the previous method and dry-form panels.

Main Methods:

Revised BMD method incorporating DMSO and P-80.
Testing of 462 Gram-positive isolates, including resistant strains.
Comparison of MIC results with previous BMD method and Sensititre dry-form panels.

Main Results:

The revised method yielded 2- to 8-fold lower MIC50 values for staphylococci and enterococci.
MIC50 values for staphylococci ranged from 0.03 to 0.06 μg/ml.
MIC50 values for E. faecium and E. faecalis were 0.03 and 0.12 μg/ml, respectively.
MIC decreases for streptococci were less significant.
High essential agreement (≥94.0%) observed with a candidate dry-form panel.

Conclusions:

The revised BMD method with DMSO and P-80 offers more accurate telavancin MIC determination.
Previous methods may have underestimated in vitro telavancin potency.
The updated method is crucial for reliable antimicrobial susceptibility testing of telavancin.