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Parasite load estimation by qPCR differentiates between asymptomatic and symptomatic infection in Indian visceral
Medhavi Sudarshan1, Shyam Sundar1
1Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221 005, UP, India.
Diagnostic Microbiology and Infectious Disease
|July 16, 2014
Abstract:
Using quantitative PCR (qPCR), we differentiated asymptomatic and symptomatic Indian Leishmania donovani infection. qPCR on blood of 40 visceral leishmaniasis, 130 endemic, and 40 non-endemic healthy controls showed 500 times less (P < .0001) parasitemia in asymptomatic compared to the symptomatic ones and threshold of 5 parasite genome/mL for the clinical disease.
