Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

3.2K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
3.2K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

13.6K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
13.6K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

6.8K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
6.8K
Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

3.4K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.4K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.4K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.4K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

64.3K
Overview
64.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Subgroup analysis of patients with Kawasaki Disease based on blood tests and cytokine profiles.

Pediatric research·2026
Same author

Hydrogen in the Earth core inferred from neutron imaging and diffraction.

Scientific reports·2026
Same author

Fused-Ring Nanothread Synthesized via High-Pressure Polymerization of Naphthalene.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Lymphoid tissue chemokines limit priming duration to preserve CD8<sup>+</sup> T cell functionality.

Science (New York, N.Y.)·2026
Same author

Homeostatic mature dendritic cells instruct fibroblast specialization via Notch2 signaling to establish T cell niches.

Immunity·2026
Same author

Preferential induction of Dt2 gene in the absence of E1 function enhances semi-determinate stem growth in soybean.

Journal of experimental botany·2026
Same journal

Optineurin restrains IL-17-associated neuroinflammation in trigeminal ganglia to preserve sensory function after ocular HSV-1 infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Related Experiment Video

Updated: Apr 27, 2026

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

31.1K

Lymph node stromal cells negatively regulate antigen-specific CD4+ T cell responses.

Jun Abe1, Shigeyuki Shichino2, Satoshi Ueha2

  • 1Department of Molecular Preventive Medicine, Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan; Japan Science and Technology Agency, Tokyo 102-8666, Japan; Theodor Kocher Institute, University of Bern, CH-3012 Bern, Switzerland;

Journal of Immunology (Baltimore, Md. : 1950)
|July 16, 2014
PubMed
Summary
This summary is machine-generated.

Lymph node stromal cells (LNSCs) respond uniquely to viral infection. Fibroblastic reticular cells (FRCs) expand slowly, and activated LNSCs present antigens, potentially regulating CD4(+) T cell responses.

More Related Videos

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
09:10

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Published on: December 16, 2013

5.3K
Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis
07:45

Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis

Published on: January 26, 2024

2.5K

Related Experiment Videos

Last Updated: Apr 27, 2026

Isolation of Murine Lymph Node Stromal Cells
05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

31.1K
Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
09:10

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Published on: December 16, 2013

5.3K
Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis
07:45

Author Spotlight: A Model to Study the Systemic and Local Dynamics of CD8+ T Cells During LN Metastasis

Published on: January 26, 2024

2.5K

Area of Science:

  • Immunology
  • Cell Biology
  • Virology

Background:

  • Lymph node stromal cells (LNSCs) are crucial for lymphocyte survival and immune tolerance.
  • LNSC responses to microbial infections remain poorly understood.

Purpose of the Study:

  • To investigate LNSC subset kinetics and function during vaccinia virus infection.
  • To elucidate the role of LNSCs in regulating T cell responses post-infection.

Main Methods:

  • Analysis of LNSC subset expansion and localization after vaccinia virus infection.
  • Assessment of major histocompatibility complex (MHC) class II expression on LNSCs.
  • Genetic ablation models to study the impact of stromal cell antigen presentation on T cell dynamics.

Main Results:

  • Distinct expansion kinetics observed among LNSC subsets, with delayed FRC expansion sustained by progenitor cells.
  • Newly generated FRCs localized to perivascular areas, with expansion occurring after viral clearance.
  • Upregulated MHC class II expression on LNSCs post-infection, which, upon genetic ablation, led to impaired CD4(+) T cell contraction.

Conclusions:

  • Activated LNSCs possess enhanced antigen-presenting capacity, acting as a brake on CD4(+) T cell responses.
  • Dysfunctional LNSC homeostasis may underlie immune deregulation in chronic viral infections and autoimmune diseases.